Mantis Viewer can generate plots of any segment features that have been calculated or imported. Segmentation data must be loaded and segment features must be present for Mantis to generate plots. The following animation gives a brief overview of generating and interacting with plots in Mantis. See below for more detailed instructions.
Once segmentation data has been loaded you can access plots by clicking the
Show Plot Pane button or by selecting the
Pop-out Plot Window entry in the
When you first load the plot view you should see a blank view with a dropdown that says
Select plot markers.... If you do not see any options when you click on the dropdown it means that segment features have not been calculated or imported.
Click the field and start typing marker names. Once you’ve selected two different channels you should be shown a scatter plot comparing the intensities for each segment.
You can click the
Controls button to the right of the marker selection field to change the type of plot, the plot statistic used, how the data is normalized, the size of dots on the plot, whether or not data from all images is shown, if the data is downsampled, or plotting the data on a log scale.
Mantis is able to generate scatter plots, contour plots, histograms, and heatmaps. For heatmaps, Mantis can summarize the data using a mean or median calculation, and it can normalize per marker or per population.
Mantis can apply a Log or ArcSinh transformation with a user supplied coefficient to the data prior to plotting.
If you have loaded segment features for a project, Mantis is able to plot segment features for all images in a project. When plotting all segment features in a project, you can plot them per image or have all of the features from non-active images collapsed into one group.
Mantis allows you to downsample the number of segments displayed on the plot. This is useful when the plot is slowed down or difficult to interpret due to a large number of data points.
You can select populations in the plot to view on the image or in other plots by using the lasso select or the box select tools.
Selecting populations is disabled when plot data is downsampled due to technical limitations with the plotting library.
You can read more about populations on the populations page